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(0)Rat Leptin ELISA Kit
Product Specifications
| Catalog # | 90040 |
| Sample Size | 5 µL |
| Sample Types | Serum, plasma, cell culture, or fluid |
| Tests | 96 wells |
| Reagents | In liquid form (except standard) |
| Sensitivity | 0.05 ng/mL |
| Assay Range | 0.2 - 12.8 ng/mL |
| Precision | Intra-assay precision CV ≤ 10.0% Inter-assay precision CV ≤ 10.0% |
| Storage | 2-8°C |
| Regulatory | Research Use Only |
| Distribution | Worldwide |
| Assay Summary |
Assay and protocol summary |
Additional Info
Recovery: When rat leptin was spiked into rat serum, the recovery of rat leptin was 100% ± 20%
Specificity:
| Substance | Reactivity |
| r-Mouse Leptin | 100% |
| r-Rat Leptin | 100% |
| r-Human Leptin | Detected* |
| Mouse Insulin | Not Detected |
| Rat C-Peptide | Not Detected |
| Rat pancreatic polypeptide | Not Detected |
| Glucagon (1-37) | Not Detected |
| Glucagon (1-29) | Not Detected |
| r-Human Insulin like growth factor-I | Not Detected |
| r-Human Insulin like growth factor-II | Not Detected |
| Human Somatostatin | Not Detected |
| *Can vary from lot to lot. Specific cross reactivity data is included with each kit | |
Typical Standard Curve:
References
| Tajaddini, Aynaz, et al. “Male Rat Offspring Are More Impacted by Maternal Obesity Induced by Cafeteria Diet than Females—Additive Effect of Postweaning Diet.” International Journal of Molecular Sciences 2022; 23: 1442. Read more |
| Nelson, Nnamdi G., et al. “Joint and Separate Exposure to Alcohol and ∆9-Tetrahydrocannabinol Produced Distinct Effects on Glucose and Insulin Homeostasis in Male Rats.” Scientific Reports 2019; 9: 12025 Read more |
| Lawson, William J., et al. "Vertical sleeve gastrectomy improves indices of metabolic disease in rodent model of surgical menopause." Menopause 2017; 24: 426-436. Read more |
| Jaimes-Hoy, Lorraine, et al. "Neonatal maternal separation alters, in a sex-specific manner, the expression of TRH, of TRH-degrading ectoenzyme in the rat hypothalamus, and the response of the thyroid axis to starvation." Endocrinology 2016; 157: 3253-3265. Read more |
| Teich, Trevor, et al. "Glucocorticoid antagonism limits adiposity rebound and glucose intolerance in young male rats following the cessation of daily exercise and caloric restriction." American Journal of Physiology - Endocrinology and Metabolism 2016; 311: E56-68. Read more |
| de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197. Read more |
| Sontam, D. M., et al. "Different short-term mild exercise modalities lead to differential effects on body composition in healthy prepubertal male rats." BioMed Research International 2015: Article ID 404201. Read more |
| Basharat, Saadia, et al. "Leptin fails to blunt the lipopolysaccharide-induced activation of the hypothalamic–pituitary–adrenal axis in rats." Journal of Endocrinology 2014; 221: 229-234. Read more |
| Liu, Xian, et al. "Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats." Physiology & Behavior 2014; 130: 99-107. Read more |
Mouse Leptin ELISA Kit