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(0)Mouse Leptin ELISA Kit
Product Specifications
| Catalog # | 90030 |
| Sample Size | 5 µL |
| Sample Types | Serum, plasma, cell culture, or fluid |
| Tests | 96 wells |
| Reagents | In liquid form (except standard) |
| Sensitivity | 0.05 ng/mL |
| Assay Range | 0.2 - 12.8 ng/mL |
| Precision | Intra-assay precision CV ≤ 10.0% Inter-assay precision CV ≤ 10.0% |
| Storage | 2-8°C |
| Regulatory | Research Use Only |
| Distribution | Worldwide |
| Assay Summary |
Assay and protocol summary |
Additional Info
Recovery: When mouse leptin was spiked into mouse serum, the recovery of mouse leptin was 100% ± 20%
Specificity:
| Substance | Reactivity |
| r-Mouse Leptin | 100% |
| r-Rat Leptin | 100% |
| r-Human Leptin | Detected* |
| Mouse Insulin | Not Detected |
| Rat C-Peptide | Not Detected |
| Rat pancreatic polypeptide | Not Detected |
| Glucagon (1-37) | Not Detected |
| Glucagon (1-29) | Not Detected |
| r-Human Insulin like growth factor-I | Not Detected |
| r-Human Insulin like growth factor-II | Not Detected |
| Human Somatostatin | Not Detected |
| *Can vary from lot to lot. Specific cross reactivity data is included with each kit | |
Typical Standard Curve:
References
| Thibonnier, Marc, and Sujoy Ghosh. “Strategy for Pre-Clinical Development of Active Targeting MicroRNA Oligonucleotide Therapeutics for Unmet Medical Needs.”International Journal of Molecular Sciences 2023; 24: 7126. Read more |
| Escalante-Covarrubias, Quetzalcoatl, et al. “Time-of-Day Defines NAD+ Efficacy to Treat Diet-Induced Metabolic Disease by Synchronizing the Hepatic Clock in Mice.” Nature Communications 2023; 14; 1685. Read more |
| Frühbeck, Gema, et al. “Increased Aquaporin-7 Expression Is Associated with Changes in Rat Brown Adipose Tissue Whitening in Obesity: Impact of Cold Exposure and Bariatric Surgery.” International Journal of Molecular Sciences 2023; 24: 3412. Read more |
| Li, Veronica L., et al. “An Exercise-Inducible Metabolite That Suppresses Feeding and Obesity.” Nature 2022; 606: 785–790. Read more |
| Çakır, Işın, et al. “Histone Deacetylase 6 Inhibition Restores Leptin Sensitivity and Reduces Obesity.” Nature Metabolism 2022; 4: 44–59. Read more |
| Plows, Jasmine F., et al. "The effects of myo-inositol and B and D vitamin supplementation in the db/+ mouse model of gestational diabetes mellitus." Nutrients 2017; 9: 141. Read more |
| McPherson, Nicole O., et al. "Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers." Scientific Reports 2016; 6: 27010. Read more |
| Dubois, Vanessa, et al. "Androgen deficiency exacerbates high fat diet0induced metabolic alterations in male mice." Endocrinology 2016; 157: 648-665. Read more |
| Clarke, Julia R., et al. "Alzheimer‐associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation." EMBO Molecular Medicine 2015; 7: 190-210. Read more |
| Chen, Yan, et al. "Enhancement of hypothalamic STAT3 acetylation by nuclear receptor Nur77 dictates leptin sensitivity." Diabetes 2015; 64: 2069-2081. Read more |
Rat Leptin ELISA Kit