Rat Leptin ELISA Kit
Product Specifications
Catalog # | 90040 |
Sample Size | 5 µL |
Sample Types | Serum, plasma, cell culture, or fluid |
Tests | 96 wells |
Reagents | In liquid form (except standard) |
Assay Range | 0.2 - 12.8 ng/mL |
Precision | Intra-assay precision CV ≤ 10.0% Inter-assay precision CV ≤ 10.0% |
Storage | 2-8°C |
Regulatory | Research Use Only |
Distribution | Worldwide |
Assay Summary |
Assay and protocol summary |
Additional Info
Recovery: When rat leptin was spiked into rat serum, the recovery of rat leptin was 100% ± 20%
Specificity:
Substance | Reactivity |
r-Mouse Leptin | 100% |
r-Rat Leptin | 100% |
r-Human Leptin | Detected* |
Mouse Insulin | Not Detected |
Rat C-Peptide | Not Detected |
Rat pancreatic polypeptide | Not Detected |
Glucagon (1-37) | Not Detected |
Glucagon (1-29) | Not Detected |
r-Human Insulin like growth factor-I | Not Detected |
r-Human Insulin like growth factor-II | Not Detected |
Human Somatostatin | Not Detected |
*Can vary from lot to lot. Specific cross reactivity data is included with each kit |
Typical Standard Curve:
References
Tajaddini, Aynaz, et al. “Male Rat Offspring Are More Impacted by Maternal Obesity Induced by Cafeteria Diet than Females—Additive Effect of Postweaning Diet.” International Journal of Molecular Sciences 2022; 23: 1442. Read more |
Nelson, Nnamdi G., et al. “Joint and Separate Exposure to Alcohol and ∆9-Tetrahydrocannabinol Produced Distinct Effects on Glucose and Insulin Homeostasis in Male Rats.” Scientific Reports 2019; 9: 12025 Read more |
Lawson, William J., et al. "Vertical sleeve gastrectomy improves indices of metabolic disease in rodent model of surgical menopause." Menopause 2017; 24: 426-436. Read more |
Jaimes-Hoy, Lorraine, et al. "Neonatal maternal separation alters, in a sex-specific manner, the expression of TRH, of TRH-degrading ectoenzyme in the rat hypothalamus, and the response of the thyroid axis to starvation." Endocrinology 2016; 157: 3253-3265. Read more |
Teich, Trevor, et al. "Glucocorticoid antagonism limits adiposity rebound and glucose intolerance in young male rats following the cessation of daily exercise and caloric restriction." American Journal of Physiology - Endocrinology and Metabolism 2016; 311: E56-68. Read more |
de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197. Read more |
Sontam, D. M., et al. "Different short-term mild exercise modalities lead to differential effects on body composition in healthy prepubertal male rats." BioMed Research International 2015: Article ID 404201. Read more |
Basharat, Saadia, et al. "Leptin fails to blunt the lipopolysaccharide-induced activation of the hypothalamic–pituitary–adrenal axis in rats." Journal of Endocrinology 2014; 221: 229-234. Read more |
Liu, Xian, et al. "Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats." Physiology & Behavior 2014; 130: 99-107. Read more |