Rat Leptin ELISA Kit

The Leptin Rat ELISA is used to quantify levels of rat leptin in serum, plasma, cell culture, and fluid using only a 5 µL sample volume.
Highlights
  • Kits use a sample volume of only 5 µL
  • High Sensitivity (200 pg/mL)
  • Dynamic Range of 0.2 - 12.8 ng/mL
  • Designed for rat samples

Product Specifications

Catalog # 90040
Sample Size 5 µL
Sample Types Serum, plasma, cell culture, or fluid
Tests 96 wells
Reagents In liquid form (except standard)
Assay Range 0.2 - 12.8 ng/mL
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide
Assay Summary Assay and protocol summary

Additional Info

Recovery:       When rat leptin was spiked into rat serum, the recovery of rat leptin was 100% ± 20%

Specificity:

Substance Reactivity
r-Mouse Leptin 100%
r-Rat Leptin 100%
r-Human Leptin Detected*
Mouse Insulin Not Detected
Rat C-Peptide Not Detected
Rat pancreatic polypeptide Not Detected
Glucagon (1-37) Not Detected
Glucagon (1-29) Not Detected
r-Human Insulin like growth factor-I Not Detected
r-Human Insulin like growth factor-II Not Detected
Human Somatostatin Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit


Typical Standard Curve:

References

Lawson, William J., et al. "Vertical sleeve gastrectomy improves indices of metabolic disease in rodent model of surgical menopause." Menopause 2017; 24: 426-436.  Read more
Jaimes-Hoy, Lorraine, et al. "Neonatal maternal separation alters, in a sex-specific manner, the expression of TRH, of TRH-degrading ectoenzyme in the rat hypothalamus, and the response of the thyroid axis to starvation." Endocrinology 2016; 157: 3253-3265.  Read more
Teich, Trevor, et al. "Glucocorticoid antagonism limits adiposity rebound and glucose intolerance in young male rats following the cessation of daily exercise and caloric restriction." American Journal of Physiology - Endocrinology and Metabolism 2016; 311: E56-68.  Read more
de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197.  Read more
Sontam, D. M., et al. "Different short-term mild exercise modalities lead to differential effects on body composition in healthy prepubertal male rats." BioMed Research International 2015: Article ID 404201.  Read more
Basharat, Saadia, et al. "Leptin fails to blunt the lipopolysaccharide-induced activation of the hypothalamic–pituitary–adrenal axis in rats." Journal of Endocrinology 2014; 221: 229-234.  Read more
Liu, Xian, et al. "Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats." Physiology & Behavior 2014; 130: 99-107.  Read more