Rat Leptin ELISA Kit

The Leptin Rat ELISA is used to quantify levels of rat leptin in serum, plasma, cell culture, and fluid using only a 5 µL sample volume.
  • Kits use a sample volume of only 5 µL
  • High Sensitivity (200 pg/mL)
  • Dynamic Range of 0.2 - 12.8 ng/mL
  • Designed for rat samples

Product Specifications

Catalog # 90040
Sample Size 5 µL
Sample Types Serum, plasma, cell culture, or fluid
Tests 96 wells
Reagents In liquid form (except standard)
Assay Range 0.2 - 12.8 ng/mL
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide
Assay Summary Assay and protocol summary

Additional Info

Recovery:       When rat leptin was spiked into rat serum, the recovery of rat leptin was 100% ± 20%


Substance Reactivity
r-Mouse Leptin 100%
r-Rat Leptin 100%
r-Human Leptin Detected*
Mouse Insulin Not Detected
Rat C-Peptide Not Detected
Rat pancreatic polypeptide Not Detected
Glucagon (1-37) Not Detected
Glucagon (1-29) Not Detected
r-Human Insulin like growth factor-I Not Detected
r-Human Insulin like growth factor-II Not Detected
Human Somatostatin Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit

Typical Standard Curve:


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Teich, Trevor, et al. "Glucocorticoid antagonism limits adiposity rebound and glucose intolerance in young male rats following the cessation of daily exercise and caloric restriction." American Journal of Physiology - Endocrinology and Metabolism 2016; 311: E56-68.  Read more
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Liu, Xian, et al. "Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats." Physiology & Behavior 2014; 130: 99-107.  Read more