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Rat Leptin ELISA Kit

Price : $385.00

Catalog #: 90040
Sample Size: 5 µL
Sample Types: Serum, plasma, cell culture, or fluid
Tests: 96 wells (8 wells x 12 modules)
Reagents: In liquid form (except standard)
Assay Range: 0.2 - 12.8 ng/mL
Assay Time: Overnight procedure
Precision: Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage: 2-8°C
Regulatory: Research Use Only
Distribution: Worldwide
View FileRat Leptin Assay Summary    

Product Details

Recovery:       When rat leptin was spiked into rat serum, the recovery of rat leptin was 100% ± 20%

Specificity:

Substance Reactivity
r-Mouse Leptin 100%
r-Rat Leptin 100%
r-Human Leptin Detected*
Mouse Insulin Not Detected
Rat C-Peptide Not Detected
Rat pancreatic polypeptide Not Detected
Glucagon (1-37) Not Detected
Glucagon (1-29) Not Detected
r-Human Insulin like growth factor-I Not Detected
r-Human Insulin like growth factor-II Not Detected
Human Somatostatin Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit


Typical Standard Curve:

Highlights:
Small Sample Volume: Only 5µl 
High Sensitivity: 200 pg/ml using 5µl sample
Precision: Intra-assay and inter-assay precision CV ≤ 10.0%

References:

 
1) Sontam, D. M., et al. "Different short-term mild exercise modalities lead to differential effects on body composition in healthy prepubertal male rats." BioMed Research International 2015: Article ID 404201.
See article
 
2) Basharat, Saadia, et al. "Leptin fails to blunt the lipopolysaccharide-induced activation of the hypothalamic–pituitary–adrenal axis in rats." Journal of Endocrinology 2014; 221: 229-234.
See article
 
3) Liu, Xian, et al. "Effects of energy status and diet on Bdnf expression in the ventromedial hypothalamus of male and female rats." Physiology & Behavior 2014; 130: 99-107.
See article
 
4) Vitzel, Kaio F., et al. "Loss of the Anorexic Response to Systemic 5-Aminoimidazole-4-Carboxamide-1-β-D-Ribofuranoside Administration Despite Reducing Hypothalamic AMP-Activated Protein Kinase Phosphorylation in Insulin-Deficient Rats." PLoS ONE 2013; 8: e71944.
See article

More references available upon request.


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