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Mouse Leptin ELISA Kit

Price : $395.00

Catalog #: 90030
Sample Size: 5 µL
Sample Types: Serum, plasma, cell culture, or fluid
Tests: 96 wells (8 wells x 12 modules)
Reagents: In liquid form (except standard)
Assay Range: 0.2 - 12.8 ng/mL
Precision: Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage: 2-8°C
Regulatory: Research Use Only
Distribution: Worldwide
View FileMouse Leptin Assay Summary    

Product Details

Recovery:       When mouse leptin was spiked into mouse serum, the recovery of mouse leptin was 100% ± 20%

Specificity:

Substance Reactivity
r-Mouse Leptin 100%
r-Rat Leptin 100%
r-Human Leptin Detected*
Mouse Insulin Not Detected
Rat C-Peptide Not Detected
Rat pancreatic polypeptide Not Detected
Glucagon (1-37) Not Detected
Glucagon (1-29) Not Detected
r-Human Insulin like growth factor-I Not Detected
r-Human Insulin like growth factor-II Not Detected
Human Somatostatin Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit



Typical Standard Curve:

Highlights:
Small Sample Volume: Only 5µL 
High Sensitivity: 200 pg/mL using 5µL sample
Precision: Intra-assay and inter-assay precision CV ≤ 10.0%

References:

 
1) Dubois, Vanessa, et al. "Androgen deficiency exacerbates high fat diet0induced metabolic alterations in male mice." Endocrinology 2016; 157: 648-665.
See article
 
2) Clarke, Julia R., et al. "Alzheimer‐associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation." EMBO Molecular Medicine 2015; 7: 190-210.
See article
 
3) Forsberg, Elisabete A., et al. "Carnosine decreases IGFBP1 production in db/db mice through suppression of HIF-1." Journal of Endocrinology 2015; 225: 159-167.
See article
 
4) Chen, Yan, et al. "Enhancement of hypothalamic STAT3 acetylation by nuclear receptor Nur77 dictates leptin sensitivity." Diabetes 2015; 64: 2069-2081.
See article

More references available upon request.


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