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Mouse C-Peptide ELISA Kit, 2nd Gen

The Mouse C-Peptide ELISA Kit, 2nd Gen is used to quantify levels of mouse c-peptide in serum, plasma, cell culture, and fluid using only a 5 µL sample volume. Then 2nd Gen kit uses a one step wash procedure, making it very convenient for users.
Highlights
  • Dynamic range of 0.1 – 6.4 ng/mL using only a 5 µL sample
  • Same day assay
  • Easy to use procedure with only one wash step
  • High sensitivity of 0.05 ng/mL using only a 5 µL sample


If you are using our kit in your publication, please reference it as Crystal Chem Cat# 63050. Please let us know, and we'll thank you with a $100 Amazon Gift card!
Price:

$495.00

Price:

$495.00

(See bulk pricing)

Bulk pricing

Buy 5
$470.25 each

Product Specifications

Catalog # 63050
Sample Size 5 µL
Sample Types Serum, plasma, cell culture, or fluid
Tests 96 wells (12 x 8 well strips)
Reagents In liquid form (except lyophilized standard)
Assay Range 0.1 - 6.4 ng/mL
Assay Time ~4 hrs
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide

Additional Info

Crystal Chem's is a worldwide leading supplier of mouse and rat ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Summary of Mouse C-Peptide ELISA Kit, 2nd Gen

Remove the Microplate (marked "MIC") from the pouch.
↓
Dispense 50 µL of Assay Diluent (marked "DL") per well.
↓
Dispense 50 µL of Assay Media (marked "MEDIA") per well.
↓
Pipette 5 µL of the sample (or working mouse c-peptide standard) per well and gently shake plate for 30 secs.
↓
Dispense 50 µL of working 1X antibody-HRP conjugate per well.
↓
Cover wells with plate seal, and then gently shake the plate for 15 secs to mix. Incubate the
microplate for 3 hours at room temp.
↓
Wash each well six times with 300 µL wash buffer.
↓
Dispense 100 µL of Substrate Solution (marked "SUB") per well.
↓
Cover wells with plate seal and incubate the microplate for 30 mins at room temp
while avoiding exposure to light.
↓
Dispense 100 µL of Stop Solution (marked "STOP") per well.
↓
Measure absorbance within 15 mins at 450nm and subtract 630nm values.
↓
Calculate mouse c-peptide concentrations using the standard curve.

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Contact us for help

sales@crystalchem.com