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Ultra Sensitive Rat Insulin ELISA Kit

Price : $370.00

Catalog #: 90060
Sample Size: 5 µL
Sample Types: Serum, plasma, cell culture, or fluid
Tests: 96 wells (8 wells x 12 modules)
Reagents: In liquid form (except standard)
Sensitivity: 0.05 ng/mL
Assay Range: Low Range Assay: 0.1 - 6.4 ng/mL
Wide Range Assay: 0.1 - 12.8 ng/mL
High Range Assay: 1 - 64 ng/mL
Precision: Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage: 2-8°C
Regulatory: Research Use Only
Distribution: Worldwide
View FileUltra Rat Assay Summary    

Product Details

Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Note: For bulk purchases, save money with our 10-kit pack.

Recovery:       When rat insulin was spiked to rat serum, the recovery of rat insulin was 100% ± 15%

Specificity:

Substance Reactivity
Rat Insulin 100%
Mouse Insulin 100%
Hamster Insulin Detected*
Rat C-peptide Not Detected
Rat pancreatic polypeptide Not Detected
Porcine Glucagon Not Detected
Human Insulin like growth factor-I Not Detected
Human Insulin like growth factor-II Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit



Typical Standard Curve:

Highlights:
Small Sample Volume: Only 5 µL
High Sensitivity: 0.05 ng/mL using 5 µL sample
Low and High Insulin Assay: Both using the same kit
Precision: Intra-assay and inter-assay precision CV ≤ 10.0%
Dynamic Range: 0.1-64.0 ng/mL using 5 µL sample
Effect of Hemolysis: Almost no effect of hemolysis tested up to 20mg/ml.

References:

 
1) de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197.
See article
 
2) Schlein, Christian, et al. "FGF21 lowers plasma triglycerides by accelerating lipoprotein catabolism in white and brown adipose tissues." Cell Metabolism 2016; 23: 441-453.
See article
 
3) Lipina, Christopher, et al. "NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids." Cellular Signalling 2015; 27: 1742-1750.
See article
 
4) Reynolds, Clare M., et al. "Maternal high‐fat diet‐induced programing of gut taste receptor and inflammatory gene expression in rat offspring is ameliorated by CLA supplementation." Physiological Reports 2015; 10: e12588.
See article

More references available upon request.


For more information including placing an order, please click here.