Ultra Sensitive Rat Insulin ELISA Kit

The Insulin Rat Ultra Sensitive ELISA is used to quantify levels of rat insulin in serum, plasma, cell culture, and fluid using only a 5 µL sample volume.
Highlights
  • Kits use only 5 µL sample
  • Dynamic range of 0.1- 64 ng/mL
  • High sensitivity (50 pg/mL) using 5µL sample

Product Specifications

Catalog # 90060
Sample Size 5 µL
Sample Types Serum, plasma, cell culture, or fluid
Tests 96 wells
Reagents In liquid form (except standard)
Sensitivity 0.05 ng/mL
Assay Range Low Range Assay: 0.1 - 6.4 ng/mL
Wide Range Assay: 0.1 - 12.8 ng/mL
High Range Assay: 1 - 64 ng/mL
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide
Assay Summary Assay and protocol summary

Additional Info

Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Note: For bulk purchases, save money with our 10-kit pack.

Highlights:

Small Sample Volume Requires only a 5 µL sample, ideal for small quantity rat samples.
Highly Sensitive and Precise This assay has a sensitivity of 0.05 ng/mL using a 5 µL sample with CV ≤ 10.0%.
Wide Dynamic Range Range of 0.1 - 64.0 ng/mL, using only a 5 µL sample. Run both low and high insulin assays using the same kit.
Specific and Fast This is an easy-to-use kit that is highly specific for rat insulin with results in under 3.5 hours.



Specificity:

Substance Reactivity
Rat Insulin 100%
Mouse Insulin 100%*
Hamster Insulin Detected*
Rat C-peptide Not Detected
Rat pancreatic polypeptide Not Detected
Porcine glucagon Not Detected
Human Insulin like growth factor-I Not Detected
Human Insulin like growth factor-II Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit



Typical Standard Curve:

References

Teich, Trevor, et al. "Curcumin limits weight gain, adipose tissue growth, and glucose intolerance following the cessation of exercise and caloric restriction in rats." Journal of Applied Physiology 2017: in press.  Read more
Thygesen, Peter, et al. "Nonclinical pharmacokinetic and pharmacodynamic characterisation of somapacitan: A reversible non-covalent albumin-binding growth hormone." Growth Hormone & IGF Research 2017; 35: 8-16.  Read more
May, Aaron A., et al. "CCK increases the transport of insulin into the brain." Physiology & Behavior 2016; 165: 392-397.  Read more
de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197.  Read more
Schlein, Christian, et al. "FGF21 lowers plasma triglycerides by accelerating lipoprotein catabolism in white and brown adipose tissues." Cell Metabolism 2016; 23: 441-453.  Read more
Lipina, Christopher, et al. "NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids." Cellular Signalling 2015; 27: 1742-1750.  Read more
Reynolds, Clare M., et al. "Maternal high‐fat diet‐induced programing of gut taste receptor and inflammatory gene expression in rat offspring is ameliorated by CLA supplementation." Physiological Reports 2015; 10: e12588.  Read more