Ultra Sensitive Rat Insulin ELISA kit
Product Specifications
Catalog # | 90060 |
Sample Size | 5 µL |
Sample Types | Serum, plasma, cell culture, or fluid |
Tests | 96 wells (12 x 8 well strips) |
Reagents | In liquid form (except standard) |
Sensitivity | 0.05 ng/mL |
Assay Range | Low Range Assay: 0.1 - 6.4 ng/mL Wide Range Assay: 0.1 - 12.8 ng/mL High Range Assay: 1 - 64 ng/mL |
Precision | Intra-assay precision CV ≤ 10.0% Inter-assay precision CV ≤ 10.0% |
Storage | 2-8°C |
Regulatory | Research Use Only |
Distribution | Worldwide |
Assay Summary |
Assay and protocol summary |
Additional Info
Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.
Note: For bulk purchases, save money with our 10-kit pack.
Highlights:
Small Sample Volume | Requires only a 5 µL sample, ideal for small quantity rat samples. |
Highly Sensitive and Precise | This assay has a sensitivity of 0.05 ng/mL using a 5 µL sample with CV ≤ 10.0%. |
Wide Dynamic Range | Range of 0.1 - 64.0 ng/mL, using only a 5 µL sample. Run both low and high insulin assays using the same kit. |
Specific and Fast | This is an easy-to-use kit that is highly specific for rat insulin with results in under 3.5 hours. |
Specificity:
Substance | Reactivity |
Rat Insulin | 100% |
Mouse Insulin | 100%* |
Hamster Insulin | Detected* |
Rat C-peptide | Not Detected |
Rat pancreatic polypeptide | Not Detected |
Porcine glucagon | Not Detected |
Human Insulin like growth factor-I | Not Detected |
Human Insulin like growth factor-II | Not Detected |
*Can vary from lot to lot. Specific cross reactivity data is included with each kit |
Typical Standard Curve:
References
Wojnacki, José, et al. “Tetraspanin-8 Sequesters Syntaxin-2 to Control Biphasic Release Propensity of Mucin Granules.” Nature Communications 2023; 14: 3710. Read more |
Yang, Jiufang, et al. “Intestinal Farnesoid X Receptor Modulates Duodenal Surface Area but Does Not Control Glucose Absorption in Mice.” International Journal of Molecular Sciences 2023; 24: 4132. Read more |
Frühbeck, Gema, et al. “Increased Aquaporin-7 Expression Is Associated with Changes in Rat Brown Adipose Tissue Whitening in Obesity: Impact of Cold Exposure and Bariatric Surgery.” International Journal of Molecular Sciences 2023; 24: 3412. Read more |
Tasić, Danica, et al. “Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function.” International Journal of Molecular Sciences 2022; 23: 9023. Read more |
Dahan, Tehila, et al. “Chronic Intermittent Hypoxia during Sleep Causes Browning of Interscapular Adipose Tissue Accompanied by Local Insulin Resistance in Mice.” International Journal of Molecular Sciences 2022; 23: 15462. Read more |
de Castro Barbosa, Thais, et al. "High-fat diet reprograms the epigenome of rat spermatozoa and transgenerationally affects metabolism of the offspring." Molecular Metabolism 2016; 5: 184-197. Read more |
Schlein, Christian, et al. "FGF21 lowers plasma triglycerides by accelerating lipoprotein catabolism in white and brown adipose tissues." Cell Metabolism 2016; 23: 441-453. Read more |
Lipina, Christopher, et al. "NEU3 sialidase as a marker of insulin sensitivity: Regulation by fatty acids." Cellular Signalling 2015; 27: 1742-1750. Read more |
Reynolds, Clare M., et al. "Maternal high‐fat diet‐induced programing of gut taste receptor and inflammatory gene expression in rat offspring is ameliorated by CLA supplementation." Physiological Reports 2015; 10: e12588. Read more |