Ultra Sensitive Mouse Insulin ELISA Kit

The Insulin Mouse Ultra Sensitive ELISA is used to quantify levels of mouse insulin in serum, plasma, cell culture, and fluid using only a 5 µL sample volume.
Highlights
  • Kits use only 5 µL sample
  • Dynamic range of 0.1- 64 ng/mL
  • High sensitivity (50 pg/mL) using 5µL sample

Product Specifications

Catalog # 90080
Sample Size 5 µL
Sample Types Serum, plasma, cell culture, or fluid
Tests 96 wells
Reagents In liquid form (except standard)
Sensitivity 0.05 ng/mL
Assay Range Low Range Assay: 0.1 - 6.4 ng/mL
Wide Range Assay: 0.1 - 12.8 ng/mL
High Range Assay: 1 - 64 ng/mL
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide
Assay Summary Assay and protocol summary

Additional Info

Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Note: For bulk purchases, save money with our 10-kit pack.

Highlights:
Small Sample Volume Requires only a 5 µL sample, ideal for small quantity mouse samples.
Highly Sensitive and Precise This assay has a sensitivity of 0.05 ng/mL using a 5 µL sample with CV ≤ 10.0%.
Wide Dynamic Range Range of 0.1 - 64.0 ng/mL, using only a 5 µL sample. Run both low and high insulin assays using the same kit.
Specific and Fast This is an easy-to-use kit that is highly specific for mouse insulin with results in under 3.5 hours.


Recovery:       When mouse insulin was spiked to mouse serum, the recovery of mouse insulin was 100% ± 15%


Specificity:

Substance Reactivity
Mouse Insulin 100%
Rat Insulin 100%*
Hamster Insulin Detected*
Rat C-peptide Not Detected
Rat pancreatic polypeptide Not Detected
Porcine glucagon Not Detected
Human Insulin like growth factor-I Not Detected
Human Insulin like growth factor-II Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit



Typical Standard Curve:

References

Mani, Bharath K., et al. "Hypoglycemic effect of combined ghrelin and glucagon receptor blockade." Diabetes 2017; 66: 1847-1857.  Read more
Marcondes, Rodrigo R., et al. "Exercise differentially affects metabolic functions and white adipose tissue in female letrozole- and dihydrotestosterone-induced mouse models of polycystic ovary syndrome." Molecular and Cellular Endocrinology 2017; 448: 66-76.  Read more
McPherson, Nicole O., et al. "An exercise-only intervention in obese fathers restores glucose and insulin regulation in conjunction with the rescue of pancreatic islet cell morphology and microRNA expression in male offspring." Nutrients 2017; 9: 122.  Read more
Thrailkill, Kathryn M., et al. "The impact of SGLT2 inhibitors, compared with insulin, on diabetic bone disease in a mouse model of type 1 diabetes." Bone 2017; 94: 141-151.  Read more
Tanigaki, Keiji, et al. "Endothelial Fcγ receptor IIB activation blunts insulin delivery to skeletal muscle to cause insulin resistance in mice." Diabetes 2016; 65: 1996-2005.  Read more
Bronsart, Laura L., et al. " A role of the adaptive immune system in glucose homeostasis."BMJ Open Diabetes Research and Care 2016; 4: e000136.  Read more
Davidson, Shawn M., et al. "Environment impacts the metabolic dependencies of ras-driven non-small cell lung cancer." Cell Metabolism 2016; 23: 517-518.  Read more
Chhabra, Kavaljit H., et al. "Hypothalamic POMC-deficiency improves glucose tolerance despite insulin resistance by increasing glycosuria." Diabetes 2016; 65: 660-672.  Read more
Nøhr, Mark K., et al. "LPS-enhanced glucose-stimulated insulin secretion is normalized by resveratrol." PLoS ONE 2016; 11: e0146840.  Read more