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Ultra Sensitive Mouse Insulin ELISA Kit

Price : $380.00

Catalog #: 90080
Sample Size: 5 µL
Sample Types: Serum, plasma, cell culture, or fluid
Tests: 96 wells (8 wells x 12 modules)
Reagents: In liquid form (except standard)
Sensitivity: 0.05 ng/mL
Assay Range: Low Range Assay: 0.1 - 6.4 ng/mL
Wide Range Assay: 0.1 - 12.8 ng/mL
High Range Assay: 1 - 64 ng/mL
Precision: Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage: 2-8°C
Regulatory: Research Use Only
Distribution: Worldwide
View FileUltra Mouse Assay Summary    

Product Details

Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.

Note: For bulk purchases, save money with our 10-kit pack.

Highlights:

Small Sample Volume Requires only a 5 µL sample, ideal for small quantity mouse samples.
Highly Sensitive and Precise This assay has a sensitivity of 0.05 ng/mL using a 5 µL sample with CV ≤ 10.0%.
Wide Dynamic Range Range of 0.1 - 64.0 ng/mL, using only a 5 µL sample. Run both low and high Insulin assays using the same kit.
Effect of Hemolysis Almost no effect of hemolysis tested up to 20mg/mL.


Recovery:       When mouse insulin was spiked to mouse serum, the recovery of mouse insulin was 100% ± 15%


Specificity:

Substance Reactivity
Mouse Insulin 100%
Rat Insulin 100%
Hamster Insulin Detected*
Rat C-peptide Not Detected
Rat pancreatic polypeptide Not Detected
Porcine glucagon Not Detected
Human Insulin like growth factor-I Not Detected
Human Insulin like growth factor-II Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit



Typical Standard Curve:

References:

 
1) Bronsart, Laura L., et al. " A role of the adaptive immune system in glucose homeostasis."BMJ Open Diabetes Research and Care 2016; 4: e000136.
See article
 
2) Davidson, Shawn M., et al. "Environment impacts the metabolic dependencies of ras-driven non-small cell lung cancer." Cell Metabolism 2016; 23: 517-518.
See article
 
3) Chhabra, Kavaljit H., et al. "Hypothalamic POMC-deficiency improves glucose tolerance despite insulin resistance by increasing glycosuria." Diabetes 2016; 65: 660-672.
See article
 
4) Nøhr, Mark K., et al. "LPS-enhanced glucose-stimulated insulin secretion is normalized by resveratrol." PLoS ONE 2016; 11: e0146840.
See article
 

More references available upon request.


For more information including placing an order, please click here.