Ultra Sensitive Mouse Insulin ELISA Kit
Product Specifications
Catalog # | 90080 |
Sample Size | 5 µL |
Sample Types | Serum, plasma, cell culture, or fluid |
Tests | 96 wells (12 x 8 well strips) |
Reagents | In liquid form (except standard) |
Sensitivity | 0.05 ng/mL |
Assay Range | Low Range Assay: 0.1 - 6.4 ng/mL Wide Range Assay: 0.1 - 12.8 ng/mL High Range Assay: 1 - 64 ng/mL |
Precision | Intra-assay precision CV ≤ 10.0% Inter-assay precision CV ≤ 10.0% |
Storage | 2-8°C |
Regulatory | Research Use Only |
Distribution | Worldwide |
Assay Summary |
Assay and protocol summary |
Additional Info
Crystal Chem's is a worldwide leading supplier of mouse and rat insulin ELISA kits. Our kits are the most sensitive on the market, and are used by leading research universities, pharma companies, and government institutions around the world.
Note: For bulk purchases, save money with our 10-kit pack.
Small Sample Volume | Requires only a 5 µL sample, ideal for small quantity mouse samples. |
Highly Sensitive and Precise | This assay has a sensitivity of 0.05 ng/mL using a 5 µL sample with CV ≤ 10.0%. |
Wide Dynamic Range | Range of 0.1 - 64.0 ng/mL, using only a 5 µL sample. Run both low and high insulin assays using the same kit. |
Specific and Fast | This is an easy-to-use kit that is highly specific for mouse insulin with results in under 3.5 hours. |
Recovery: When mouse insulin was spiked to mouse serum, the recovery of mouse insulin was 100% ± 15%
Specificity:
Substance | Reactivity |
Mouse Insulin | 100% |
Rat Insulin | 100%* |
Hamster Insulin | Detected* |
Rat C-peptide | Not Detected |
Rat pancreatic polypeptide | Not Detected |
Porcine glucagon | Not Detected |
Human Insulin like growth factor-I | Not Detected |
Human Insulin like growth factor-II | Not Detected |
*Can vary from lot to lot. Specific cross reactivity data is included with each kit |
Typical Standard Curve:
References
Wang, Dongdong, et al. “GDF15 Promotes Weight Loss by Enhancing Energy Expenditure in Muscle.” Nature 2023; 619: 143–150. Read more |
Slieker, Roderick C., et al. “Identification of Biomarkers for Glycaemic Deterioration in Type 2 Diabetes.” Nature Communications 2023; 14: 2533. Read more |
Inia, José A., et al. “Semaglutide Has Beneficial Effects on Non-Alcoholic Steatohepatitis in Ldlr-/-.Leiden Mice.” International Journal of Molecular Sciences2023; 24: 8494. Read more |
Li, Xingyu, et al. “SIRT2 Deficiency Aggravates Diet-Induced Nonalcoholic Fatty Liver Disease through Modulating Gut Microbiota and Metabolites.” International Journal of Molecular Sciences 2023; 24: 8970. Read more |
Costa-Machado, Luis Filipe, et al. “Peripheral Modulation of Antidepressant Targets MAO-B and GABAAR by Harmol Induces Mitohormesis and Delays Aging in Preclinical Models.” Nature Communications 2023; 14: 2779. Read more |
Mani, Bharath K., et al. "Hypoglycemic effect of combined ghrelin and glucagon receptor blockade." Diabetes 2017; 66: 1847-1857. Read more |
Marcondes, Rodrigo R., et al. "Exercise differentially affects metabolic functions and white adipose tissue in female letrozole- and dihydrotestosterone-induced mouse models of polycystic ovary syndrome." Molecular and Cellular Endocrinology 2017; 448: 66-76. Read more |
McPherson, Nicole O., et al. "An exercise-only intervention in obese fathers restores glucose and insulin regulation in conjunction with the rescue of pancreatic islet cell morphology and microRNA expression in male offspring." Nutrients 2017; 9: 122. Read more |
Thrailkill, Kathryn M., et al. "The impact of SGLT2 inhibitors, compared with insulin, on diabetic bone disease in a mouse model of type 1 diabetes." Bone 2017; 94: 141-151. Read more |
Tanigaki, Keiji, et al. "Endothelial Fcγ receptor IIB activation blunts insulin delivery to skeletal muscle to cause insulin resistance in mice." Diabetes 2016; 65: 1996-2005. Read more |