Rat Insulin ELISA Kit
Product Specifications
Catalog # | 90010 |
Sample Size | 5 µL |
Sample Types | Serum, plasma, fluid |
Tests | 96 wells (12 x 8 well strips) |
Assay Range | 0.156 - 10.0 ng/mL using 5 µL sample 0.039 - 2.5 ng/mL using 20 µL sample |
Precision | Intra-assay precision CV ≤ 10.0% Inter-assay precision CV ≤ 10.0% |
Storage | 2-8°C |
Regulatory | Research Use Only |
Distribution | Worldwide |
Assay Summary |
Assay and protocol summary |
Additional Info
We also offer the Ultra Sensitive Rat Insulin ELISA Kit that has a faster assay time and better sensitivity.
Recovery: When rat insulin was spiked to rat serum, the recovery of rat insulin was 100% ± 10%
Specificity:
Substance | Reactivity |
Rat Insulin | 100% |
Mouse Insulin | 105% |
Porcine Insulin | Detected* |
Hamster Insulin | Detected* |
Human Insulin (recombinant) | Detected* |
Human Proinsulin | Detected* |
Rat C-peptide | Not Detected |
Rat pancreatic polypeptide | Not Detected |
Porcine glucagon | Not Detected |
Human Insulin like growth factor-I | Not Detected |
Human Insulin like growth factor-II | Not Detected |
*Can vary from lot to lot. Specific cross reactivity data is included with each kit |
Optional products to use with this kit:
References
Henderson, Mark J., et al. “A Target-Agnostic Screen Identifies Approved Drugs to Stabilize the Endoplasmic Reticulum-Resident Proteome.” Cell Reports 2021; 35: 109040. Read more |
Fernandez, Diego Carlos, et al. “Retinal Innervation Tunes Circuits That Drive Nonphotic Entrainment to Food.” Nature 2020: 581; 194–198. Read more |
Dijkstra, Dorieke J., et al. “Mid-Gestation Low-Dose LPS Administration Results in Female-Specific Excessive Weight Gain upon a Western Style Diet in Mouse Offspring.” Scientific Reports 2020: 10; 19618. Read more |
Bernier, Michel, et al. “Disulfiram Treatment Normalizes Body Weight in Obese Mice.” Cell Metabolism 2020; 32: 203-214. Read more |
Lalwani, Amit, et al. “β Cell Hypoxia-Inducible Factor-1α Is Required for the Prevention of Type 1 Diabetes.” Cell Reports 2019; 27: 2370-2384. Read more |
Marwitz, Shannon E., Lauren N. Woodie, and Sarah N. Blythe. "Western-style diet induces insulin insensitivity and hyperactivity in adolescent male rats." Physiology & Behavior 2015; 151: 147-154. Read more |
Paula, Flavia MM, et al. "Exercise increases pancreatic β-cell viability in a model of type 1 diabetes through IL-6 signaling." The FASEB Journal 2015; 29: 1805-1816. Read more |
Christensen, Camilla S., et al. "Skeletal muscle to pancreatic β-cell cross-talk: The effect of humoral mediators liberated by muscle contraction and acute exercise on β-cell apoptosis." The Journal of Clinical Endocrinology & Metabolism 2015; 100: E1289-E1298. Read more |
Vernochet, Cecile, et al. "Adipose tissue mitochondrial dysfunction triggers a lipodystrophic syndrome with insulin resistance, hepatosteatosis, and cardiovascular complications." The FASEB Journal 2014; 28: 4408-4419. Read more |