Rat Insulin ELISA Kit

The Insulin Rat ELISA is used to quantify levels of rat insulin in serum, plasma, and fluid using only a 5 µL sample volume.
Highlights
  • Kits use a sample size of only 5 μL
  • Wide dynamic range of 0.156 to 10.0 ng/mL
  • High Sensitivity (0.156 ng/mL)

Product Specifications

Catalog # 90010
Sample Size 5 µL
Sample Types Serum, plasma, fluid
Tests 96 wells
Assay Range 0.156 - 10.0 ng/mL using 5 µL sample
0.039 - 2.5 ng/mL using 20 µL sample
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Regulatory Research Use Only
Distribution Worldwide
Assay Summary Assay and protocol summary

Additional Info

Recovery:       When rat insulin was spiked to rat serum, the recovery of rat insulin was 100% ± 10%

Specificity:

Substance Reactivity
Rat Insulin 100%
Mouse Insulin 105%
Porcine Insulin Detected*
Hamster Insulin Detected*
Human Insulin (recombinant) Detected*
Human Proinsulin Detected*
Rat C-peptide Not Detected
Rat pancreatic polypeptide Not Detected
Porcine glucagon Not Detected
Human Insulin like growth factor-I Not Detected
Human Insulin like growth factor-II Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit


Optional products to use with this kit:
 

Mouse Insulin Standard

References

Marwitz, Shannon E., Lauren N. Woodie, and Sarah N. Blythe. "Western-style diet induces insulin insensitivity and hyperactivity in adolescent male rats." Physiology & Behavior 2015; 151: 147-154.  Read more
Paula, Flavia MM, et al. "Exercise increases pancreatic β-cell viability in a model of type 1 diabetes through IL-6 signaling." The FASEB Journal 2015; 29: 1805-1816.  Read more
Christensen, Camilla S., et al. "Skeletal muscle to pancreatic β-cell cross-talk: The effect of humoral mediators liberated by muscle contraction and acute exercise on β-cell apoptosis." The Journal of Clinical Endocrinology & Metabolism 2015; 100: E1289-E1298.  Read more
Vernochet, Cecile, et al. "Adipose tissue mitochondrial dysfunction triggers a lipodystrophic syndrome with insulin resistance, hepatosteatosis, and cardiovascular complications." The FASEB Journal 2014; 28: 4408-4419.  Read more