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Rat Insulin ELISA Kit

Price : $370.00

Catalog #: 90010
Sample Size: 5 µL
Sample Types: Serum, plasma, fluid
Tests: 96 wells
Assay Range: 0.156 - 10.0 ng/mL using 5 µL sample
0.039 - 2.5 ng/mL using 20 µL sample
Precision: Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage: 2-8°C
Regulatory: Research Use Only
Distribution: Worldwide

Product Details

Recovery:       When rat insulin was spiked to rat serum, the recovery of rat insulin was 100% ± 10%

Specificity:

Substance Reactivity
Rat Insulin 100%
Mouse Insulin 105%
Porcine Insulin Detected*
Hamster Insulin Detected*
Human Insulin (recombinant) Detected*
Human Proinsulin Detected*
Rat C-peptide Not Detected
Rat pancreatic polypeptide Not Detected
Porcine glucagon Not Detected
Human Insulin like growth factor-I Not Detected
Human Insulin like growth factor-II Not Detected
*Can vary from lot to lot. Specific cross reactivity data is included with each kit



Typical Standard Curve:

Highlights:
Small Sample Volume: Only 5µL
Wide Range: 0.156 to 10.0 ng/mL using a 5µL sample
High Sensitivity: 0.156 ng/mL using a 5µL sample
Precision:Intra-assay and inter-assay precision CV ≤ 10%

Optional products to use with this kit: 

Mouse Insulin Standard

References:

1) Marwitz, Shannon E., Lauren N. Woodie, and Sarah N. Blythe. "Western-style diet induces insulin insensitivity and hyperactivity in adolescent male rats." Physiology & Behavior 2015; 151: 147-154.
See article
 
2) Paula, Flavia MM, et al. "Exercise increases pancreatic β-cell viability in a model of type 1 diabetes through IL-6 signaling." The FASEB Journal 2015; 29: 1805-1816.
See article
 
3) Christensen, Camilla S., et al. "Skeletal muscle to pancreatic β-cell cross-talk: The effect of humoral mediators liberated by muscle contraction and acute exercise on β-cell apoptosis." The Journal of Clinical Endocrinology & Metabolism 2015; 100: E1289-E1298.
See article
 
4) Vernochet, Cecile, et al. "Adipose tissue mitochondrial dysfunction triggers a lipodystrophic syndrome with insulin resistance, hepatosteatosis, and cardiovascular complications." The FASEB Journal 2014; 28: 4408-4419.
See article

More references available upon request.


For more information including placing an order, please click here.