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Rat Leptin ELISA (EIA) Kit

      Catalog#
          90040
Rat Leptin ELISA Kit

 
Sample Size : 5µl



Samples:

Serum, plasma, or fluid
Tests: 96 wells (8 wells x 12 modules)
Reagents: In liquid form (except standard)
Assay Range: 0.2 - 12.8 ng/ml

Assay Time: Overnight procedure
Precision: Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%

Recovery: When rat leptin was spiked into rat serum, the recovery of rat leptin was 100% ± 20%
Specificity:
Substance Reactivity
r-Rat Leptin 100%
r-Mouse Leptin 100%*
r-Human Leptin 40%*
Mouse Insulin Not detected
Rat C-Peptide Not detected
Rat pancreatic polypeptide Not detected
Glucagon (1-37) Not detected
Glucagon (1-29) Not detected
r-Human Insulin like growth factor-I Not detected
r-Human Insulin like growth factor-II Not detected

*Can vary from lot to lot. Specific cross reactivity data is included with each kit

Typical Standard Curve:
Highlights: Small Sample Volume: Only 5µl

High Sensitivity: 200 pg/ml using 5µl sample

Precision: Intra-assay and inter-assay precision CV ≤ 10.0%

Summary of Assay
Rat Leptin ELISA Kit (Catalog# 90040)
 
Affix the antibody-coated microplate to the frame
Wash each well two times with wash buffer*
Dispense 45 µl of sample diluent per well
Dispense 50 µl guinea pig anti-mouse leptin serum per well
Pipette 5 µl of the sample (or working rat leptin standard) to the well
Incubate the microplate overnight (16-20 hours) at 4° C.
Wash each well five times with wash buffer*
Dispense 100 µl of anti-guinea Pig IgG enzyme conjugate per well
Incubate the microplate 3 hours at 4°C.
Wash each well seven times with wash buffer*
Dispense 100 µl enzyme substrate solution per well
Incubate microplate for 30 minutes at room temperature while avoiding exposure to light
Stop the enzyme reaction by adding 100µl enzyme reaction stop solution per well
Measure A450 and subtract A630 values within 30 minutes
Calculate leptin concentration using the standard curve
 
      * Each well should be washed with 300µl of wash buffer. Aspirate the wells completely so all                               
        excess solution is removed.
 
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