Rat Leptin ELISA (EIA) Kit

Catalog#
90040

Rat Leptin ELISA Kit

Sample Size :

5µl

Samples:

Serum, plasma, or fluid

Tests:

96 wells (8 wells x 12 modules)

Reagents:

In liquid form (except standard)

Assay Range:

0.2 - 12.8 ng/ml

Assay Time:

Overnight procedure

Precision:

Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%

Recovery:

When rat leptin was spiked into rat serum, the recovery of rat leptin was 100% ± 20%

Specificity:

Substance	Reactivity
r-Rat Leptin	100%
r-Mouse Leptin	100%*
r-Human Leptin	40%*
Mouse Insulin	Not detected
Rat C-Peptide	Not detected
Rat pancreatic polypeptide	Not detected
Glucagon (1-37)	Not detected
Glucagon (1-29)	Not detected
r-Human Insulin like growth factor-I	Not detected
r-Human Insulin like growth factor-II	Not detected

*Can vary from lot to lot. Specific cross reactivity data is included with each kit

Typical Standard Curve:

Highlights:

Small Sample Volume: Only 5µl

High Sensitivity: 200 pg/ml using 5µl sample

Precision: Intra-assay and inter-assay precision CV ≤ 10.0%

Summary of Assay Rat Leptin ELISA Kit (Catalog# 90040)

Affix the antibody-coated microplate to the frame

Wash each well two times with wash buffer*

Dispense 45 µl of sample diluent per well

Dispense 50 µl guinea pig anti-mouse leptin serum per well

Pipette 5 µl of the sample (or working rat leptin standard) to the well

Incubate the microplate overnight (16-20 hours) at 4° C.

Wash each well five times with wash buffer*

Dispense 100 µl of anti-guinea Pig IgG enzyme conjugate per well

Incubate the microplate 3 hours at 4°C.

Wash each well seven times with wash buffer*

Dispense 100 µl enzyme substrate solution per well

Incubate microplate for 30 minutes at room temperature while avoiding exposure to light

Stop the enzyme reaction by adding 100µl enzyme reaction stop solution per well

Measure A₄₅₀ and subtract A₆₃₀ values within 30 minutes

Calculate leptin concentration using the standard curve

* Each well should be washed with 300µl of wash buffer. Aspirate the wells completely so all
excess solution is removed.

Crystal Chem Inc. Tel: (630) 889-9003 Fax: (630) 889-9021 E-mail: sales@crystalchem.com