Insulin ELISA Kit

The Human Insulin ELISA is a highly sensitive assay used to quantify levels of human insulin in serum and plasma.
Highlights
  • ~100% Reactivity to insulin and most analogs
  • Very low (1.2%) reactivity to proinsulin
  • Very low (< 5%) reactivity to mouse/rat insulin
  • Fast assay time (2 hrs 15 mins)
  • Wide dyanamic range of 0.9 - 220 mU/L
For bulk purchases

Save money with our 10-kit pack

Product Specifications

Catalog # 90095
Sample Size 25 µL
Sample Types Serum, plasma
Tests 96 wells (12 x 8 well strips)
Assay Range 0.9 - 220 mU/L
Conversion: 1 mU/L = 34.8 pg/mL
Precision Intra-assay precision CV ≤ 10.0%
Inter-assay precision CV ≤ 10.0%
Storage 2-8°C
Specificity Insulin.......................100%
Insulin Lispro.............108%
Insulin Aspart..............94%
Insulin Glargine..........104%
Proinsulin...................1.2%
C-peptide......................0%
Mouse/Rat Insulin........<5%
Synonyms Human Insulin ELISA
Assay Summary Assay and protocol summary
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Additional Info

Highlights:

Very specific ~100% Reactivity to insulin and most analogs
No reactivity to C-peptide
Very low (1.2%) reactivity to proinsulin
Very low (< 5%) reactivity to mouse/rat insulin
Wide Dynamic Range 0.9 - 220 mU/L
Specific and Fast This is an easy-to-use kit that is highly specific for human insulin with results in under 2.5 hours.


Note: For bulk purchases, save money with our 10-kit pack.


For more information including placing an order, please click here

References

Cao, Minghui, et al. “Cancer-Cell-Secreted Extracellular Vesicles Suppress Insulin Secretion through MiR-122 to Impair Systemic Glucose Homeostasis and Contribute to Tumour Growth.” Nature Cell Biology 2022; 24: 954–967.  Read more
Wang, Jiani, et al. “Elafin Inhibits Obesity, Hyperglycemia, and Liver Steatosis in High-Fat Diet-Treated Male Mice.” Scientific Reports 2020; 10.  Read more
Sun, Da, et al. “MiR‐802 Causes Nephropathy by Suppressing NF‐ΚB‐Repressing Factor in Obese Mice and Human.” Journal of Cellular and Molecular Medicine 2019; 23: 2863–2871.  Read more
Harney, D., et al."Small protein enrichment assay enables the rapid, unbiased analysis of over 100 low abunhdance factors from human plasma." Molecular and Cellular Proteomics 2019; in press.  Read more
Crew, C., et al. "SREBP-regulated adipocyte lipogenesis is dependent on substrate availability and redox modulation of mTORC1." JCI Insight 2019; 4(15): e129397.  Read more
Sun, D., et al. "MiR-802 cuases nephropathy by suppressing NF-ΚB-repressing factor in obese mice and human." J of Cellular and Molecular Medicine 2019; 23(4): 2863-2871.  Read more
Jeffery, N, et al. "The Species Origin of the Cellular Microenvironment Influences Markers of Beta Cell Fate and Function in EndoC-BH1 cells." Experimental Cell Research 2017; 361: 284-291  Read more