Gluten (Gliadin) ELISA Kit

The Gluten (Gliadin) ELISA Kit is used to quantify levels of gluten (gliadin) in raw and processed food, and uses an innovative extraction method to more accurately detect allergen levels.
Highlights
  • Improved detection of allergens in raw and processed food
  • High sensitivity (0.31 ppm)
  • Dynamic range detectable by this kit is 0.31 – 20 ppm.
  • Common extraction solution among all kits
  • High recovery of allergen

Product Specifications

Catalog # M2103
Sample Size 1 g
Tests 96 wells
Sensitivity 0.26 ppm Gluten
0.31 ppm Wheat
Assay Range Standard Assay:
0.26 - 17 ppm Gluten
0.31 - 20 ppm Wheat

High Range Assay:
1.05 - 68 ppm Gluten
1.24 - 80 ppm Wheat
Storage 2-8°C
Methodology Sandwich ELISA, Quantitative
Specificity Gliadin
Assay Summary Assay and protocol summary
Key Publication Journal of Food Protection

Additional Info

Crystal Chem offers an innovative Gluten (Gliadin) ELISA kit for the improved detection of gluten in raw & processed foods. The kit is manufactured by Morinaga Institute and utilizes a proprietary extraction solution to effectively measure wheat (and accordingly gluten) from both raw and processed foods.

The gluten kit is 10 lab validated and is cited by the FDA as a method that will be used to determine compliance with its recent Gluten Free Food Labeling Rule finalized in August 2014.

Useful Publications

 
1)Panda, Rakhi, et al. "Detection and Quantification of Gluten during the Brewing and Fermentation of Beer Using Antibody-Based Technologies." Journal of Food Protection 78(6), 1167-1177.

Excerpt: “The sensitivity of the MIoBS [Morinaga] ELISA (0.25 ppm) enabled the reliable detection of gluten throughout the manufacturing process, including fermentation, when the initial concentration of 20 μg/ml dropped to 2 μg/ml. The R5 antibody–based and G12 antibody–based sandwich ELISAs were unable to reliably detect gluten, initially at 20 μg/ml, after the onset of production..”

See article
Crystal Chem also offers food allergen kits for Egg, Milk, Buckwheat, and Peanut. For more information on the gluten kit or other allergen kits, including placing an order, please click here.