E. Coli Host Cell Protein (HCP) ELISA Kits

Our new line of generic E. Coli HCP kits utilize a new approach towards generic ELISA. Rather than offering a single assay, four assays are provided each employing a different anti-HCP antibody. Customers can then choose the assay which gives the best coverage for their process.

Using this new approach, our kits have demonstrated better recovery than Cygnus kits in over 65% of samples tested.

Read more about our new approach and why it works better 

How E.Coli|360-HCP ELISA works

How the antibodies were generated

E.coli|360-HCP Assay is based on W3110 and BL21(DE3) cell lines which were fermented in different culture media and conditions resulting in four antigen preparations with distinct HCP patterns. By immunizing goats with these antigens, BioGenes has developed a panel of four different HCP ELISAs (type A, C, D, and E) that together build up the enhanced generic E.coli|360-HCP Assay. {NOTE: Type E replaces the older Type B kit.}

A high specificity for the antigen in all four assays was determined by Coomassie staining of the E.coli HCP antigen and 2D western blotting with the respective antibodies.

Product specs and other Info

Product specifications:

• LOD between 0.5 – 1.0 ng/mL
• LOQ between 2 – 3 ng/mL
• Working range 2 – 100 ng/mL

Other information:

• Item list and catalog numbers
• Product Insert
• Article in BioProcess Yearbook
• MSDS